在生物醫(yī)學(xué)和分子生物學(xué)研究中，CD4分子因其在人類免疫缺陷病毒(HIV)中的作用而備受關(guān)注。不幸的是，CD4充當(dāng)HIV的高親和力受體，病毒利用它進(jìn)入宿主T細(xì)胞。在發(fā)生HIV感染時(shí)，CD4+T細(xì)胞的數(shù)量逐漸減少，使宿主容易受到許多病原體的侵害。在臨床診斷中，CD4計(jì)數(shù)常用于評(píng)估感染HIV的患者免疫系統(tǒng)的健康狀況。

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CD4單克隆抗體經(jīng)常用于流式細(xì)胞術(shù)和免疫熒光應(yīng)用，以識(shí)別表達(dá)CD4分子的免疫細(xì)胞亞群。已知表達(dá)CD4表面標(biāo)志物的細(xì)胞包括胸腺細(xì)胞、成熟T細(xì)胞的特定亞群、單核細(xì)胞、巨噬細(xì)胞、樹突細(xì)胞和NKT細(xì)胞。

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艾美捷iFluor?標(biāo)記CD4抗體推薦：

名稱?貨號(hào)?Ex (nm)?Em (nm)

iFluor 350 Anti-human CD4 Antibody?10040010?344?448

iFluor 405 Anti-human CD4 Antibody?10040020?402?425

iFluor 430 Anti-human CD4 Antibody?10040030?433?495

iFluor 450 Anti-human CD4 Antibody?10040040?451?502

iFluor 488 Anti-human CD4 Antibody?10040050?491?516

iFluor 514 Anti-human CD4 Antibody?10040060?527?554

iFluor 532 Anti-human CD4 Antibody?10040070?543?563

iFluor 546 Anti-human CD4 Antibody?10040080?541?557

iFluor 555 Anti-human CD4 Antibody?10040090?556?569

iFluor 560 Anti-human CD4 Antibody?100400A0?559?571

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艾美捷mFluor染料：

熒光團(tuán)?Ex (nm)?Em (nm)?ε?Φ?CF 260 nm?CF 280 nm

mFluor UV375?354?388?35,000?0.94?0.099?0.138

mFluor Violet 450?406?445?25,000?0.92?0.338?0.078

mFluor UV460?362?456?15,000?0.86?0.35?0.134

mFluor Violet 500?426?497?35,000?0.81?0.769?0.365

mFluor Violet 510?409?504?30,000?0.86?0.464?0.366

mFluor Violet 540?400?532?15,000?0.64?1.392?0.529

mFluor Blue 570?552?564?120,000?0.08?0.228?0.179

mFluor Green 620?521?617?50,000?0.06?0.895?0.569

mFluor Yellow 630?546?625?110,000?0.01?0.283?0.413

mFluor Red 700?657?694?250,000?0.029?0.135?0.127

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艾美捷?Alexa Fluor染料：

熒光團(tuán)?Ex (nm)?Em (nm)?ε?Φ?CF 280 nm

Alexa Fluor 350?343?441?19,000?N/D?0.19

Alexa Fluor 488?499?520?73,000?0.92?0.11

Alexa Fluor 555?553?568?155,000?0.1?0.08

Alexa Fluor 594?590?618?92,000?0.66?0.56

Alexa Fluor 647?650?671?270,000?0.33?0.03

Alexa Fluor 680?681?704?183,000?0.36?0.05

Alexa Fluor 700?696?719?205,000?0.25?0.07

Alexa Fluor 750?752?776?290,000?0.12?0.04

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艾美捷經(jīng)典染料：

熒光團(tuán)?Ex (nm)?Em (nm)?ε?Φ?CF 280 nm

Pacific Blue?404?455?46,000?0.78?0.2

FITC?491?516?73,000?0.79?0.254

Pacific Orange?400?551?25,000?N/D?0.6

TRITC?544?570?85,000?0.1?0.34

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艾美捷藻膽蛋白和串聯(lián)染料：

熒光團(tuán)?Ex (nm)?Em (nm)?ε?激光?(nm)

PE?566?574?1,960,000?488 nm, 532 nm, 561-568 nm

PE-Texas Red?567?615?1,960,000?488 nm, 532 nm, 561-568 nm

PE-Alexa Fluor 610?567?627?1,960,000?488 nm, 532 nm, 561-568 nm

PE-Cy5?565?666?1,960,000?488 nm, 532 nm, 561-568 nm

PE-iFluor 647?569?666?1,960,000?488 nm, 532 nm, 561-568 nm

PE-Alexa Fluor 700?566?721?1,960,000?488 nm, 532 nm, 561-568 nm

PE-Cy7?566?778?1,960,000?488 nm, 532 nm, 561-568 nm

PE-iFluor 750?566?778?1,960,000?488 nm, 532 nm, 561-568 nm

APC?651?660?700,000?633-647 nm

APC-iFluor 700?651?710?700,000?633-647 nm