MQAE；Cl-（氯離子）熒光探針
MQAE是一種氯離子熒光探針，可用于測(cè)量細(xì)胞內(nèi)和細(xì)胞外氯化物濃度（最大吸收/發(fā)射：350/460 nm）。MQAE 比?36Cl 同位素示蹤法和微電極法更靈敏，更有選擇性。
圖1.MQAE化學(xué)結(jié)構(gòu)
MQAE基礎(chǔ)信息
中文名：氯離子熒光探針;熒光氯離子指示劑
英文名：MQAE； Fluorescent chloride ion indicator;1-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide
CAS號(hào)：162558-52-3
結(jié)構(gòu)式：CCOC(=O)C[n+]1cccc2c1ccc(c2)OC.[Br-]
分子式：C14H16BrN3
分子量：326.18600
熔點(diǎn)：177-179° C (lit.)
溶解度：DMF: 12 mg/mL DMSO: 20 mg/mL Ethanol: 16 mg/mL PBS (pH 7.2): 10 mg/mL
性狀：淺黃色至卡其色固體
儲(chǔ)藏條件： 4°C,-4℃下存儲(chǔ)更優(yōu)
使用案例
圖2.Fluorescence imaging with the dye, MQAE, of Cl i ? in Amphiuma red blood cells. The changes in the distribution of intracellular Cl ? between the nucleus and cytoplasm are quickly evident ( ? 5 sec) upon the sequential changes in Cl o . Because the fluorescence of the dye is not affected by NO 3 ? , we could rapidly and reversibly monitor fluorescence changes in Cl i ? mediated by the exchange, via Band 3, of intracellular Cl i ? with NO 3 ? by exchanging the 101 mM Cl o ? solution with a solution containing 101 mM NO 3 ? . Here the compositions of the two solutions were the same, with equal osmolalities, except for the change of anions. All changes in fluorescence (images are comprised of pseudocolors) were stable for at least 5 min. It should be emphasized that the fluorescence of the dye is decreased (quenched) by Cl ? ). In A , with the cells suspended in a Cl o ? -free NO 3 ? solution, the fluorescence intensity of the cytoplasm is high. In B , upon exchange of the medium with 101 mM Cl o ? , the relative fluorescence of the cytoplasm is markedly decreased due to Cl ? entry into the cell. As shown in C and D , these changes.
——biofount范德生物是（貨號(hào)：YZM018891）
