3D細胞培養(yǎng)是一種在三維空間內(nèi)培養(yǎng)細胞的技術(shù)，與傳統(tǒng)的2D細胞培養(yǎng)不同。在3D細胞培養(yǎng)中，細胞可以自然地形成復雜的三維結(jié)構(gòu)，如球體、管狀結(jié)構(gòu)和多層結(jié)構(gòu)等，這種結(jié)構(gòu)更接近于生物體內(nèi)的真實環(huán)境。3D細胞培養(yǎng)技術(shù)已經(jīng)被廣泛應用于癌癥研究、組織工程、藥物篩選等領域。它可以提供更準確的生物學信息，例如細胞-細胞相互作用、細胞-基質(zhì)相互作用以及化學梯度的影響。此外，3D細胞培養(yǎng)還可以更好地模擬生物體內(nèi)的微環(huán)境，從而更好地預測藥物反應和疾病發(fā)展。 ??

?

用于控制質(zhì)粒DNA或siRNA遞送的生物材料可以提供靶向轉(zhuǎn)基因表達（過度表達或阻斷）的基本工具，將為基因或細胞治療提供新的視角。艾美捷?OZ Biosciences-3D細胞培養(yǎng)專用轉(zhuǎn)染試劑，針對基質(zhì)膠和水凝膠優(yōu)化！

?

3D基質(zhì)類型：

艾美捷?3D細胞培養(yǎng)支架- 3D Scaffolds(#TF20250)

Collagen-based Scaffolds Collagen-derived Scaffolds Hyaluronic Acid MillicellTM (PTFE) Cell culture insert (Millipore) PCL，Polycaprolactone PEG，Poly(Ethylene Glycol) PLGA，Poly(lactic-co-glycolic acid) PS，Poly(Styrene) PU，Poly(Urethane)

3D水凝膠- 3D Hydrogels

Matrigel（BD）Collagen-Based Hydrogels Collagen-Derived Hydrogels Hyaluronic Acid Gelatin Extracellular Matrix Fibrin / Fibronectin Fibrinogen Laminin PEGylated hydrogels

?

發(fā)表文章：

細胞名稱?核酸類型?產(chǎn)品?參考文章

CLU-301?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HEK-293?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MDCK?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MEF?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1?siRNA?3D-Fect?Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

3T3?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

AdSC?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

BMSC?3D Transfection DNA?3D-FectIN?Sukarto A., Eur Cells and Mat. 2011; 22(4):28.

Chondrocytes?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa?3D Transfection siRNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Mesenchymal Stem Cells?mRNA?3D-FectIN?Ledo AM. Acta Biomater . 2020 May 15;S1742-7061(20)30224-5.

Mesenchymal Stem Cells?mRNA?3D-FectIN?Ledo AM., Biomaterials. 2020 Jul;247:120016.

Mesenchymal Stem Cells?3D Transfection DNA?3D-FectIN?Plank C., Eur. J. Nanomed. 2012;4(1):17–32

Mesenchymal Stem Cells?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

NIH-3T3?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RAW 264.7?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1?siRNA?3D-FectIN?Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

ATDC5?siRNA?si3D-FectIN?Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.

Chondrocytes?siRNA?si3D-FectIN?Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.

?

3D-Fect Transfection Reagent

?

專門開發(fā)用于直接轉(zhuǎn)染在?3D 支架中培養(yǎng)的細胞。用3D-Fect / DNA復合物激活的基于支架的3D矩陣被細胞定植，隨后在更自然的環(huán)境中原位轉(zhuǎn)染。該方法可以研究組織工程和再生，腫瘤侵襲，神經(jīng)分化，細胞極化，組織形成，定植，神經(jīng)突生長... ??

?

結(jié)果展示：

3D-Fect 允許將多種細胞（細胞系和原代細胞）轉(zhuǎn)染到 3D 支架中：基于膠原蛋白和膠原蛋白衍生的支架預加載由 3D-Fect（比例 4：1）和 1 μg pVectOZ-GFP（綠色圖片）或 RFP 質(zhì)粒（紅色圖片）形成的復合物。然后接種細胞并在48小時后在熒光顯微鏡下觀察